TIANamp Bacteria Genomic DNA Kit is based on the silica membrane technology and a special buffer system for extracting gDNA extraction from a wide range of Gram negative and Gram-positive bacteria. This kit can also be used to purify genomic DNA from pathogenic microbes in food samples, such as Staphylococcus aureus, Vibrio cholerae, hemorrhagic Escherichia coli O157: H7, Listeria monocytogenes, Salmonella, Enterobacter sakazakii, etc. Up to 10 - 40 μg genomic DNA can be rapidly purified from 1-5 ml of culture. Genomic DNA purified by the TIANamp Bacteria DNA Kit is suitable for a variety of downstream applications, including PCR amplification, restriction endonuclease digestion and Southern Blot.
Store at room temperature (15-25℃)
■ Genomic DNA can be purified from Gram-negative bacteria within one hour. ■ The high pure gDNA can be used directly in downstream experiments, such as PCR amplification, restriction endonuclease digestion and Southern Blot.
Genomic DNA purification from Gram-negative and Gram-positive bacteria Genomic DNA purification from pathogenic bacteria in food
Ethanol (96% -100%); For Gram-positive bacteria: Lysozyme & Lysozyme buffer (20 mM Tris, 2mM Na2-EDTA , 1.2% Triton pH 8.0)