miRcute miRNA Isolation Kit is uniquely designed to purify cellular small (20-200 nt) RNA molecules, including miRNA, siRNA and snRNA. The Kit can also be used to purify total RNA from samples. The Kit utilizes a silica-based two-column system to enrich small RNA from various sample sources. From sample lysate, RNAs greater than ~ 200 nt are bound to the first column. The flow-through, enriched with small RNA molecules, is mixed with a higher concentration of ethanol to increase the binding affinity of small RNA to the membranes of the second column. Bound small RNA molecules are then eluted, ready-to-use, and free of large RNA molecules that can inhibit expression analysis of small RNA molecules, Purified RNA is suitable for different downtown applications.
■ Efficiency: efficient enrichment of micro Rna and Rnas < 200 nucleotides ■ convenience: effective purification of micro Rna and total Rna ■ Simplity: the procedure is quick and convenient in about 1 hour. ■ High Performance: High-purity Rna suitable for all downstream applications
Chloroform, Ethanol (96% -100%)
Buffer mZ should be stored dry at 2-8℃; other Reagents at room temperature.