RNAprep pure Kit (For Cell / Bacteria) provides a fast, simple, and costeffective method for purification of total RNA from cultured cells and bacteria samples by using effective spin column and unique buffer system. The kit includes RNase-free spin column CR3 for purifying high-quality RNA by using silica-membrane technology. High-quality total RNA can be obtained in 30-40 minutes with high-purity and is free from protein and genomic DNAcontamination.
■ Optimized buffers and protocols for cultured cells and bacteria samples make the process simple and convenient. ■ Unique DNase I minimizes genomic DNA contamination. ■ Unique RNase-free Filtration Columns CS eliminates other contaminations. ■ High-purity ready-to-use RNA, suitable for sensitive downstream applications. ■ No phenol / chloroform extraction, no LiCl and ethanol precipitation, no CsCl gradients centrifugation.
β-mercaptoethanol, Ethanol (96% -100%)
■ RT-PCR ■ Northern blot, Dot blot ■ Real-time RT-PCR ■ Chip Analysis ■ In vitro translation, poly A screening
Dnase Ⅰ, Buffer RDD and Rnase-free ddH2o (tubular) should be stored at 2-8℃. other reagents can be stored at room temperature.