EndoFree Maxi Plasmid Kit is based on silica membrane technology to extract endotoxin-free plasmid DNA. This kit provides a unique endotoxin removal process to generate high-quality plasmid DNA for use in demanding downstream applications. It is designed to isolate plasmid DNA of the highest purity from 100 - 200 ml of bacterial culture and can yield up to 1.5 mg transfection-grade plasmid DNA.
■ 200 μg -1.5 mg plasmid DNA yield in one hour with high proportion of supercoiled structures. ■ Low endotoxin level and high purity of plasmid DNA are ensured by unique buffer system and CP6 spin columns. ■ Excellent transfection efficiency, suitable for transfection experiment of most cell lines. ■ Wide range of applications, suited for restriction endonuclease digestion, transformation, sequencing, gene therapy, cell microscopy, gene silencing and transfection experiments.